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AudioSpike - The software tool for electrophysiological measurements using audio interfaces

AudioSpike is a software package for electrophysiological measurements using acoustic stimulation for Microsoft® Windows®. Instead of proprietary hardware for recording electrode signals, AudioSpike uses standard sound cards for acoustic stimulation and recording of electrode signals. The recorded spikes can be analyzed in real-time using multi-channel cluster analysis and PSTH selection.
Interfaces from/to MATLAB® or GNU Octave to set up the measurement tasks and to read raw measurement data and evaluation data into the workspace are delivered with AudioSpike.

Advantages of AudioSpike

  • multi-channel spike detection (recording)
  • multi-channel acoustic stimulation
  • real-time GUI interfaces for spike selection, cluster analysis, PSTH, vector strength and much more
  • Special search modes with real-time spike train and spike visualization
  • Calibration and frequency compensation interfaces
  • in-situ calibration procedure
  • no proprietary hardware required: use standard sound cards for spike recording
  • interfaces from/to MATLAB®/Octave
  • support for offline analysis: re-analysis of spike trains after setting new detection thresholds, cluster selections

For a list of some references, please click here.

Info

AudioSpike is designed to set up multi-channel electrophysiological measurements with acoustic stimulation with powerful real-time spike detection and analysis "on-the-fly". It uses standard sound cards under Microsoft® Windows® for stimulation and spike recording. Costly proprietary hardware is no longer required for electrode signal acquisition.
For information on technical requirements, see the Technology section.

AudioSpike's main features include:

  • Multi-channel spike detection (recording) with sample-accurate synchronization of all channels. The number of channels is limited only by the number of Souncard channels
  • Multi-channel acoustic stimulation with sample-accurate synchronization of all channels
  • Real-time GUI interfaces:
    • Spike train plots with visual threshold adjustment
    • Spike waveform plots
    • Cluster plots (X-Y plots of spike parameters) with spike selection for PSTH generation
    • PSTH with choices for parameter plots
    • Parameter plots: X-Y bubble plots plotting the number of spikes within PSTH choices for stimulus-parameter combinations
    • Period histogram and vector strength plots
  • Two search modes:
    • Free search mode: stimulus selection (frequency and level) during cell search. Spiketrains and spikes are plotted live (with optional averaging mode)
    • List search: load a stimulus list and play stimuli in a loop until a new one is selected
  • Easy-to-use calibration and frequency equalization interfaces for level calibration
  • Additional equalization mode for loading custom frequency responses (impulse responses)
  • In-situ calibration method
  • Interfaces from/to MATLAB®/Octave
    • Generate your measurement paradigms and your stimuli in MATLAB®/Octave and transfer them to AudioSpike
    • Load measurement and analysis data back into MATLAB®/Octave, including spiketrain raw data, spike waveforms, and complete spike and stimulus parameter information for each spike
  • Monitor function: 'listen' to electrode signals during search
  • Detailed documentation with examples and how-to's

Support

In this section you will find support resources such as frequently asked questions (FAQ), version history and support contact information.
If your question is not answered in the manuals or FAQ, please contact our support by sending an email to support@hz-ol.de senden.


Please always include the following information:

  • Detailed description of the problem, with a screenshot if possible
  • AudioSpike version number
  • Microsoft® Windows® version
  • Sound card manufacturer, model, driver and firmware version
  • If you have problems with the connection from/to MATLAB® or GNU Octave, please also include the version number of MATLAB® / GNU Octave.

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